NuMega operates a Perkin Elmer PE-SCIEX API-150 mass spectrometer equipped with an electrospray ionization source. Electrospray, developed in 1980s, is a mild ionization technique, which is useful for nonvolatile and unstable materials. Normally the parent ions are the dominant signals. Positive mode usually detects M+H+, M+Na+, and M+K+, while negative mode detects M+H-, and M+Cl-. Our detection range on the instrument is 30 – 3000 m/z. We report both the positive and negative ionization scans of your sample.
Mass Spectrometry (mass spec) is an analytic technique used to measure the mass of molecular ions. The mass spec operates by injecting a dilute sample of your molecule which then undergoes electrospray ionization (ESI) usually through protonation or cationization. The ions are dispersed in an electric field generating highly charged droplets from which solvent vaporizes. The resulting molecular ions are then introduced into the mass spectrometer. The ions are mass filtered by a quadrupole and are detected using a channeltron electron multiplier. The signal processor delivers the observed spectrum with the recorded mass-to-charge ratio.
Check out our Mass Spec LinkedIn Showcase page here