Instruments for NMR Spectroscopy

NMR Testing Service, Bruker NMR | NuMega Resonance Labs

NuMega operates two 500 MHz Bruker NMR spectrometers, DRX 500 and AV-500. We have a variety of highly sensitive probes that can be interchangably fitted into our magnet. The diversity of both instruments enables us to carry out various experiments with a fast turnaround time. We perform the following experiments:

  • 1D- H-1, C-13, P-31 and other nuclei, such as B-10, F19, Si-29, V-51, Sn-119, etc.
  • 1D NOE, Water Supression, Decoupling, VT, Quantitative NMR, DEPT, DEPTQ, INEPT
  • 2D-COSY, NOESY, TOCSY, ROESY, HSQC, HMBC, and other pulse sequences.
  • We also design NMR experiments, develop test methods and interpret your data. If you want us to perform an experiment not listed, please contact us to see how we can assist you.

NMR Probes Available at NuMega

  • Inverse Probe: 5mm, Triple Channel, High Sensitive H-1, Pulsed Field Gradient Coil
  • Broad Band Probe: 5 mm, Capable of being tuned to P-31, C-13, N-15, B-10, B-11, V-51, Sn-119, 0-17, Li-7, etc., Pulsed Field Gradient Coil
  • Broad Band Probe: 10mm, 4x volume-strong signal

Instruments for Mass Spectroscopy

Mass Spec | NuMega Resonance Labs

NuMega operates a Perkin Elmer PE-SCIEX API-150 mass spectrometer equipped with an electrospray ionization source. Electrospray, developed in 1980s, is a mild ionization technique, which is useful for nonvolatile and unstable materials. Normally the parent ions are the dominant signals. Positive mode usually detects M+H+, M+Na+, and M+K+, while negative mode detects M+H-, and M+Cl-. Our detection range on the instrument is 30 – 3000 m/z. We report both the positive and negative ionization scans of your sample.

Instruments for Elemental Analysis

Elemental Analysis | NuMega Resonance Labs

NuMega operates a Perkin Elmer PE2400-Series II, CHNS/O analyzer for elemental analysis. The CHNS content is determined using the Pregl-Dumas method where your sample is prepared, introduced into the combustion chamber and burned at high temperature 925°F under pure oxygen. The resulting gas mixture and helium carrier gas pass through various reductive and catalytic zones to convert the gas mixture into CO2, H2O, N2, and SO2. The gasses are separated using gas chromatography and quantified with a thermal conductivity detector.

Instruments for Optical Rotation

Polarimeter, Polarimetry, Specific Optical Rotation, Optical Rotation | NuMega Resonance Labs

NuMega operates a Jasco P-2000 polarimeter for the measurement of optical activity in chiral molecules. Our current light source for the instrument is a sodium lamp with a 589 nm wavelength filter. The polarimeter operates by directing light through a polarized lens and through the sample cell to quantify how much the molecule in solution bends in relation to the light source. All samples that rotate to the right or dextrorotary are designated with a (+) for clockwise motion. All samples that rotate to the left or levorotatory are designated with a (-) for counter-clockwise motion. Our instrument is equipped with a temperature controlled chamber capable of maintaining temperatures anywhere from 15-40°C. We have 2 cell sizes: 3.5 x 100mm with a capacity for 3 mL of solvent and 10 x 100mm with a capacity for 10 mL solvent to accommodate the needs of your experiment. Our report includes the specific optical rotation, concentration, temperature, and path length of the cell.